Hinterdorfer P., van Oijen A. (Eds.) Handbook of Single-Molecule Biophysics

Springer Verlag, 2009. - 626 p.This book will introduce the reader to the different classes of single-molecule approaches developed over recent years and how these methods can be applied to understand biological systems. Broadly, single-molecule biophysical techniques can be separated into two classes of methods: one that allows us to visualize single molecules, the other to manipulate them. The potential of imaging the emission of single fluorophores has led to many exciting developments that allow us to study biomacromolecules, their movement, their interactions, and their cellular context. Biochemical and genetic methods have been developed that allow the very specific and precise fluorescent labeling of biomacromolecules. Optical excitation and detection methods have been introduced that allow the visualization of individual, fluorescently labeled molecules with high spatial and temporal resolution.The Handbook provides a comprehensive overview for a broad audience of researchers in
biophysics, molecular biology, chemistry, and related areas, as well as for advanced undergraduate
and graduate students.Single-molecule fluorescence tracking
Single molecule studies in the membrane
Single-molecule imaging in live cells
Superresolution imaging
Fluorescence Resonance Energy Transfer
Single-molecule enzymology
Rotary proteins
Fluorescence Correlation Spectroscopy
Advanced Concepts of Fluorescence Fluctuation Spectroscopy
Nucleic-acid analysis at the single-molecule level
Nanopores: Geneneration and Single-Molecule Applications
Optical trapping
Magnetic tweezing
Protein/DNA unfolding
Single molecule recognition
Nano-scale AFM imaging
High Speed AFM
Recognition imaging
Atomic force microscopy of protein-protein interactions
A New Approach to Analysis of Single Molecule Force Measurements
Single Molecule Recognition: Extracting Information from Individual Binding Events and Their Correlation