Glick B.R., Pasternak J.J. Molecular Biotechnology: Principles and Applications of Recombinant DNA

ASM Press, 2003. - 760 p. - Completely revised, updated, and expanded, the 3rd edition of Molecular Biotechnology: Principles and Applications of Recombinant DNA covers the underlying scientific principles and deals extensively with the many industrial, agricultural, pharmaceutical, and biomedical applications of recombinant DNA technology.
The 3rd edition offers greatly expanded coverage of directed mutagenesis and protein engineering, therapeutic agents, and genetic engineering of plants. Updated chapters reflect recent developments in biotechnology and the societal issues related to it, such as cloning, gene therapy, and patenting and releasing genetically engineered organisms. Over 500 figures, including 200 that are new in this edition, illustrate all key concepts. "Milestones" summarize important research papers in the history of biotechnology and their effects on the field. As with previous editions, the authors clearly explain all concepts and techniques to provide maximum understanding of the subject, avoiding wherever possible confusing scientific jargon and excessive detail. Each chapter concludes with a summary, references, and review questions.
Ideally suited as a text for third- and fourth-year undergraduates as well as graduate students, this book is also an excellent reference for health professionals, scientists, engineers, or attorneys interested in biotechnology.
In this Edition:
- Significantly updated to reflect the advances of the past five years.
- Various recent examples replace those from the mid-1990s and earlier.
- Over 200 new figures illustrate the added concepts and principles.
- Covers the major advances since publication of the previous edition.Preface to the First Edition
Fundamentals of Molecular Biotechnology
The Molecular Biotechnology Revolution
Molecular Biotechnology Biological Systems
DNA, RNA, and Protein Synthesis
Recombinant DNA Technology
Chemical Synthesis, Sequencing, and Amplification of DNA
Manipulation of Gene Expression in Prokaryotes
Heterologous Protein Production in Eukaryotic Cells
Directed Mutagenesis and Protein Engineering
Molecular Biotechnology of Microbial Systems
Molecular Diagnostics
Therapeutic Agents
Vaccines
Synthesis of Commercial Products by Recombinant Microorganisms
Bioremediation and Biomass Utilization
Plant Growth-Promoting Bacteria
Microbial Insecticides
Large-Scale Production of Proteins from Recombinant Microorganisms
Eukaryotic Systems
Genetic Engineering of Plants: Methodology
Genetic Engineering of Plants: Applications
Transgenic Animals
Human Molecular Genetics
Regulating and Patenting Molecular Biotechnology
Regulating the Use of Biotechnology
Patenting Biotechnology Inventions