Elsevier, 2009. - 534 p. - The 33rd volume of Laboratory Techniques in Biochemistry and Molecular Biology Series reviews the techniques Forster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields. This book has no too much math equations, and the content is easy to understand. The perspective and future development in FRET-FLIM technologies are excellent, and this book is helpful for those who are interested in biophysics, biomedical and biophotonics but not major in biology in university.