Shivashankar G.V. (Ed.). Nuclear Mechanics and Genome Regulation

Elsevier, 2010. - 367 p. (Methods in Cell Biology, Volume 98) - In recent years new discoveries have made this an exciting and important field of research. This comprehensive volume presents comprehensive chapters and detailed background information for researchers working with in the field of Nuclear Mechanics and Genome Regulation.
* Both classic and state-of-the-art methods readily adaptable and designed to last the test of time
* Relevant to clinicians and scientists working in a wide range of fieldsContents
Cell Nucleus: Organization & MechanoBiology
Fluorescence Fluctuation Microscopy to reveal 3D-Architecture and Function in the Cell Nucleus
Studying histone modifications and their genomic functions by employing chromatin immunoprecipitation and immunoblotting
Dynamic organization of chromatin assembly & transcription factories in living cells
Manipulation & Isolation of Single Cells & Nuclei
Beyond lamins: other structural components of the nucleoskeleton
Altered mechanical properties of the nucleus in disease
Theoretical concepts and models of cellular mechanosensing
Impact of Nuclear Mechanics on Function
Mechanical Induction of Gene Expression in Connective Tissue Cells
Physical plasticity of the Nucleus and its manipulation
Prestressed Nuclear Organization in Living Cells
Nanotopography/mechanical induction of stem-cell differentiation
Mechanical Induction in Embryonic Development and Tumour Growth: Integrative Cues through Molecular to Multi-cellular Interplay, and Evolutionary Perspectives
Informatics based analysis of mechanosignaling in the laminopathies
Autosomal Dominant Leukodystrophy caused by lamin B1 duplications: A clinical and molecular case study of altered nuclear function and disease