Soloviev M. (Ed.) Nanoparticles in Biology and Medicine: Methods and Protocols

Humana Press, 2012. - 544 p.
The modern fascination with micro- and nano-sized materials can actually be traced back further to the 1960s and ‘70s when the first few reported attempts were made to use nanoparticles for controlled drug delivery. In Nanoparticles in Biology and Medicine: Methods and Protocols, experts in the field present a wide range of methods for synthesis, surface modification, characterization, and application of nano-sized materials (nanoparticles) in life science and medical fields, mostly for drug delivery. The methods presented cover all stages of nanoparticle manufacturing, modification, analysis, and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Nanoparticles in Biology and Medicine: Methods and Protocols will help the beginner become familiar with this fascinating field and will provide scientists at all levels of expertise with easy-to-follow practical advice needed to make, modify, and analyze nanoparticles of their choice and to use them in a wide range of biomedical and pharmaceutical applications, including functional protein studies, drug delivery, immunochemistry, imaging, and many others.Noble Metal Nanoparticles: Synthesis and Applications
Procedures for the Synthesis and Capping of Metal Nanoparticles
Preparation of Concentrated Colloids of Gold Core-Silica Shell Nanoparticles for Biomedical Applications
Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis
Nanoparticle Antibody Conjugates for Use in Competitive Lateral Flow Assays
Gold Nanoparticles for Immunogold Localization of Antigens in Plant Tissues
RNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Colour Multiplexing and Application to Cancer Diagnostics
Effect of Gold Nanoparticle Conjugation on the Activity and Stability of Functional Proteins
Semiconductor Nanoparticles: Synthesis and Applications
Synthesis of Semiconductor Nanoparticles
Size-Selective Synthesis of Ultrasmall Hydrophilic CdSe Nanoparticles in Aqueous Solution at Room Temperature
Synthesis and Functionalization of CdSe/ZnS QDs Using the Successive Ion Layer Adsorption Reaction and Mercaptopropionic Acid Phase Transfer Methods
In Vivo, Site-Specific, Covalent Conjugation of Quantum Dots to Proteins Via Split-Intein Splicing
Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis
The Use of Quantum Dots for Immunochemistry Applications
Quantum Dots for Labelling Live Cells
Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots
The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption, Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry
Magnetic Nanoparticles: Synthesis and Applications
Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging
Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging
Non-Invasive Quantification of Magnetic Nanoparticles by Means of Magnetorelaxometry
Synthesis, Application, and Tracking of Magnetic Carbon-Coated Nanoparticles in Plants
Organic, Polymeric, Biomimetic, and Biocompatible Nanoparticles and Their Applications
Synthesis of Biocompatible Gelatinated Thioglycolic Acid-Capped CdTe Quantum Dots (Jelly Dots)
Preparation and Characterization of Biomimetic Nanoparticles for Drug Delivery
Preparation of Nanoparticles by Spray-Drying and Their Use for Efficient Pulmonary Drug Delivery
Synthesis and Evaluation of Airway Targeted PLGA Nanoparticles for Drug Delivery in Obstructive Lung Diseases
Preparation of siRNA-Encapsulated PLGA Nanoparticles for Sustained Release of siRNA and Evaluation of Encapsulatiuon Efficiency
Synthesis of Poly (2-hydroxyethyl methacrylate) (PHEMA) Based Nanoparticles for Biomedical and Pharmaceutical Applications
of Lipid:Peptide:DNA (LPD) Nanoparticles and Their Use for Gene Transfection
Crotamine: A Novel Cell-Penetrating Polypeptide Nanocarrier with Potential Anti-Cancer and Biotechnological Applications
Elastin-Based Nanoparticles for Delivery of Bone Morphogenetic Proteins
Synthesis, Surface Modification, Characterization, and Biomedical In Vitro Applications of Organically Modified Silica (ORMOSIL) Nanoparticles
Extraction of Organic Nanoparticles from Plants
Nano-Toxicity and Nano-Safety
Assessment of Cytotoxicity of Carbon Nanoparticles Using 3-(4,5-Dimethylthiazol-2-yl)-5-(3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium (MTS) Cell Viability Assay
Luciferase Reporter System for Studying the Effect of Nanoparticles on Gene Expression
Single Cell Gel Electrophoresis Assay (Comet Assay) for Evaluating Nanoparticles-Induced DNA Damage in Cells
Assessment of Toxicity of Nanoparticles Using Insects as Biological Models
Evaluation of Cytotoxicity of 3-Mercaptopropionic Acid-Modified Quantum Dots on Medicago sativa Cells and Tissues
Nanoparticles Characterization
Scanning Transmission Electron Microscopy Methods for the Analysis of Nanoparticles
Total Internal Reflection Microscopy (TIRF) for Real-Time Imaging of Nanoparticle-Cell Plasma Membrane Interaction
Microscopy Imaging Methods for the Detection of Silver and Titanium Nanoparticles Within Cells
Detection of TiO2 Nanoparticles in Cells by Flow Cytometry
NanoParticle Tracking Analysis for the Multiparameter Characterization and Counting of Nanoparticle Suspensions
Relevance of Two-Dimensional Brownian Motion Dynamics in Applying Nanoparticle Tracking Analysis
Nanoparticle Bridges for Studying Electrical Properties of Organic Molecules